Get clearer answers with accurate, reproducible Western blots and many other assays.
Do more with digital fluorescence
Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.
In-Cell Western™ Assay
EMSA / Gel Shift Assay
NIR Fluorescent Western Blot
Go where your experiments take you
Western blots, cell-based assays, protein arrays, gel shift assays, tissue section imaging, and more are at your fingertips with near-infrared (NIR) fluorescence on the Odyssey CLx.
“We are finding that we can use the Odyssey CLx for just about everything protein-related we do in the lab.”
See the full story in a single image
See both strong and faint bands clearly in a single image acquired on the Odyssey CLx, with great sensitivity and no image saturation. Multiplex with two fluorescent colors to see even more data.
One digital image file contains all your data so you can see both strong and faint bands clearly in a single image, without image saturation, “blowout”, or sacrificing sensitivity. Increase the consistency and reproducibility of your results by capturing a single image with the Odyssey CLx, rather than comparing multiple exposures captured under variable conditions.
“The superior dynamic range and sensitivity allows me to confidently report my data.”
Powerful, precise laser excitation and specialized optics enable the Odyssey CLx to provide high signal-to-noise ratios and outstanding image quality.
See multiple targets on the same blot
With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels.
Use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from you blot. View, adjust, and analyze your results as a merged image, or as separate 700nm and 800nm channel images in pseudo-color or grayscale. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.
“The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.”
ㅇㅇㅇㅇ– Bond et al (2008) Biol Proced Online. 10:20-28.
See your strong bands accurately
Get deeper data capacity with digital fluorescence and the unprecedented linear dynamic range of the Odyssey CLx, and never lose data because of image saturation
Never lose your data because of image saturation. Digital fluorescent detection with the Odyssey CLx shows your strong signals as they really are. Film and CCD imagers only show you part of the picture, because data are lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands.
“When saturated, film exposures can also hide sample-to-sample variations in high-abundance proteins.”
ddddJanes (2015) Science Signaling 8 (371) : rs2
Acquisition and analysis software for the Odyssey CLx
Pair your Odyssey CLx Imager with Image Stuido Software for streamlined image acquisition and organization and with new Empiria Studio Software for analysis of your Western blots.